ssr_primer
Primer Details for TRINITY_DN72376_c0_g4_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACCACCCGTGATGTCAGC | TAGGAGCGTTGCATGGGAAG | Start: 369 |
| Tm | 60.037 | 60.108 | End: 517 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CCGTGATGTCAGCAAGGCTA | TAGGAGCGTTGCATGGGAAG | Start: 376 |
| Tm | 60.108 | 60.108 | End: 517 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | CGCACGATTCATGGTCAAGC | ATCTTCGGCACCTTGACCTG | Start: 1121 |
| Tm | 60.248 | 60.036 | End: 1344 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | GTCGGCTGGATTCATCCCTC | GCTGACATCACGGGTGGTAA | Start: 218 |
| Tm | 60.25 | 60.037 | End: 388 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | GTCGGCTGGATTCATCCCTC | TGATTGGCTGGATGCTGGTT | Start: 218 |
| Tm | 60.25 | 59.96 | End: 359 |
| Size | 20 | 20 | 142 |
(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACCACCCGTGATGTCAGC | TAGGAGCGTTGCATGGGAAG | Start: 369 |
| Tm | 60.037 | 60.108 | End: 517 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CCGTGATGTCAGCAAGGCTA | TAGGAGCGTTGCATGGGAAG | Start: 376 |
| Tm | 60.108 | 60.108 | End: 517 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | CGCACGATTCATGGTCAAGC | ATCTTCGGCACCTTGACCTG | Start: 1121 |
| Tm | 60.248 | 60.036 | End: 1344 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | GTCGGCTGGATTCATCCCTC | GCTGACATCACGGGTGGTAA | Start: 218 |
| Tm | 60.25 | 60.037 | End: 388 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | GTCGGCTGGATTCATCCCTC | TGATTGGCTGGATGCTGGTT | Start: 218 |
| Tm | 60.25 | 59.96 | End: 359 |
| Size | 20 | 20 | 142 |
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