Primer Details for TRINITY_DN72353_c1_g4_i1 | |||
|---|---|---|---|
| (T)21: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTAGTGTACCTAAGTGTAAACGA | CGCGAAGGAGATGTAGGACC | Start: 120 |
| Tm | 59.527 | 59.969 | End: 247 |
| Size | 25 | 20 | 128 |
| Primer 2 details | |||
| Sequence | TGCTAGTGTACCTAAGTGTAAACGA | GTGTCTCGCGAAGGAGATGT | Start: 120 |
| Tm | 59.527 | 59.829 | End: 253 |
| Size | 25 | 20 | 134 |
| Primer 3 details | |||
| Sequence | TGCTAGTGTACCTAAGTGTAAACG | CGCGAAGGAGATGTAGGACC | Start: 120 |
| Tm | 58.297 | 59.969 | End: 247 |
| Size | 24 | 20 | 128 |
| Primer 4 details | |||
| Sequence | TGCTAGTGTACCTAAGTGTAAACG | GTGTCTCGCGAAGGAGATGT | Start: 120 |
| Tm | 58.297 | 59.829 | End: 253 |
| Size | 24 | 20 | 134 |
| Primer 5 details | |||
| Sequence | GCTAGTGTACCTAAGTGTAAACGA | CGCGAAGGAGATGTAGGACC | Start: 121 |
| Tm | 58.057 | 59.969 | End: 247 |
| Size | 24 | 20 | 127 |
