ssr_primer
Primer Details for TRINITY_DN72329_c1_g3_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTACGTTAACCAAGCGT | TCATGCATGGAGCTTGGTGT | Start: 1002 |
| Tm | 59.968 | 59.961 | End: 1107 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | GGAGGATGAGATCGAAGGCG | ACTAGGGAACTCGGTGTCGA | Start: 227 |
| Tm | 60.039 | 59.963 | End: 362 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | ACCGTACGTTAACCAAGCGT | GGAAGCGTCCAGTAATGCCT | Start: 1002 |
| Tm | 59.968 | 60.108 | End: 1274 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | ACACCAAGCTCCATGCATGA | GGAAGCGTCCAGTAATGCCT | Start: 1088 |
| Tm | 59.961 | 60.108 | End: 1274 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | AGGAAGCGTCTTCGTTACGG | CGCCTTCGATCTCATCCTCC | Start: 19 |
| Tm | 60.109 | 60.039 | End: 246 |
| Size | 20 | 20 | 228 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGTACGTTAACCAAGCGT | TCATGCATGGAGCTTGGTGT | Start: 1002 |
| Tm | 59.968 | 59.961 | End: 1107 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | GGAGGATGAGATCGAAGGCG | ACTAGGGAACTCGGTGTCGA | Start: 227 |
| Tm | 60.039 | 59.963 | End: 362 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | ACCGTACGTTAACCAAGCGT | GGAAGCGTCCAGTAATGCCT | Start: 1002 |
| Tm | 59.968 | 60.108 | End: 1274 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | ACACCAAGCTCCATGCATGA | GGAAGCGTCCAGTAATGCCT | Start: 1088 |
| Tm | 59.961 | 60.108 | End: 1274 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | AGGAAGCGTCTTCGTTACGG | CGCCTTCGATCTCATCCTCC | Start: 19 |
| Tm | 60.109 | 60.039 | End: 246 |
| Size | 20 | 20 | 228 |
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