ssr_primer
Primer Details for TRINITY_DN72326_c1_g1_i7 |
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| (T)10gttttgtttttttttg(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCAGAAACGCCTACCTC | AGGCCCTCATTTTCTGACCG | Start: 1089 |
| Tm | 60.037 | 60.035 | End: 1297 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
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| Sequence | ACAGCAGAAACGCCTACCTC | TGGCTCGCATGAATGTGTCT | Start: 1089 |
| Tm | 60.037 | 60.036 | End: 1239 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
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| Sequence | ACCTGAAGGGAAAGGCCATG | AGGCCCTCATTTTCTGACCG | Start: 1037 |
| Tm | 59.96 | 60.035 | End: 1297 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | ACCTGAAGGGAAAGGCCATG | TGGCTCGCATGAATGTGTCT | Start: 1037 |
| Tm | 59.96 | 60.036 | End: 1239 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
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| Sequence | TCCTTCTGGTGTGATGTGCC | CCATCGAAGCAGTGAAAGCG | Start: 1920 |
| Tm | 59.963 | 59.904 | End: 2186 |
| Size | 20 | 20 | 267 |
(GT)11aatttacctggacatatgtaagtcatgattttcagttacctgtgataatctt(TTTA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCAGAAACGCCTACCTC | AGGCCCTCATTTTCTGACCG | Start: 1089 |
| Tm | 60.037 | 60.035 | End: 1297 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | ACAGCAGAAACGCCTACCTC | TGGCTCGCATGAATGTGTCT | Start: 1089 |
| Tm | 60.037 | 60.036 | End: 1239 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | ACCTGAAGGGAAAGGCCATG | AGGCCCTCATTTTCTGACCG | Start: 1037 |
| Tm | 59.96 | 60.035 | End: 1297 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | ACCTGAAGGGAAAGGCCATG | TGGCTCGCATGAATGTGTCT | Start: 1037 |
| Tm | 59.96 | 60.036 | End: 1239 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
|---|
| Sequence | TCCTTCTGGTGTGATGTGCC | CCATCGAAGCAGTGAAAGCG | Start: 1920 |
| Tm | 59.963 | 59.904 | End: 2186 |
| Size | 20 | 20 | 267 |
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