ssr_primer
Primer Details for TRINITY_DN72319_c0_g1_i1 |
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| (A)10cacgtaaattt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCCCCAATAACGGATTCT | CTAATCCTCGGCGCCTCAAA | Start: 23 |
| Tm | 59.815 | 60.179 | End: 141 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | ACGGATTCTCTTTTCGGCGA | CTAATCCTCGGCGCCTCAAA | Start: 34 |
| Tm | 59.754 | 60.179 | End: 141 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | GGCCCCCAATAACGGATTCT | TCCTCGGCGCCTCAAATTAA | Start: 23 |
| Tm | 59.815 | 59.749 | End: 137 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
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| Sequence | ACGGATTCTCTTTTCGGCGA | TCCTCGGCGCCTCAAATTAA | Start: 34 |
| Tm | 59.754 | 59.749 | End: 137 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
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| Sequence | AATTCCCCACCTTCTTCGGG | CTAATCCTCGGCGCCTCAAA | Start: 0 |
| Tm | 59.668 | 60.179 | End: 141 |
| Size | 20 | 20 | 142 |
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