ssr_primer
Primer Details for TRINITY_DN72278_c1_g3_i5 |
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| (T)43gta(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCTACCCCCTAAATCCG | GGTCTATGCGGCAATTTCGG | Start: 238 |
| Tm | 59.892 | 59.692 | End: 343 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
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| Sequence | GTGGCTACCCCCTAAATCCG | GTCTATGCGGCAATTTCGGC | Start: 238 |
| Tm | 59.892 | 60.317 | End: 342 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
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| Sequence | CCTAAATCCGGCCCTGTTGT | TGTGGACAGCGACAGTTTTCT | Start: 248 |
| Tm | 60.034 | 60.135 | End: 427 |
| Size | 20 | 21 | 180 |
| Primer 4 details |
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| Sequence | TGCGGGACAAGTTTTCAAACC | ACAACAGGGCCGGATTTAGG | Start: 14 |
| Tm | 59.865 | 60.034 | End: 267 |
| Size | 21 | 20 | 254 |
| Primer 5 details |
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| Sequence | AGAAAACTGTCGCTGTCCACA | TGCCAGAAGTTCGGTGATCC | Start: 407 |
| Tm | 60.135 | 60.036 | End: 663 |
| Size | 21 | 20 | 257 |
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