Primer Details for TRINITY_DN72271_c3_g1_i1 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATGATCCCGTAGAAAGAAAGT | AGGTTAAGGCCGTTTTCCCT | Start: 63 |
| Tm | 57.975 | 59.224 | End: 198 |
| Size | 23 | 20 | 136 |
| Primer 2 details | |||
| Sequence | GGATGATCCCGTAGAAAGAAAGT | AGGTTAAGGCCGTTTTCCCTT | Start: 63 |
| Tm | 57.975 | 59.854 | End: 198 |
| Size | 23 | 21 | 136 |
| Primer 3 details | |||
| Sequence | GGATGATCCCGTAGAAAGAAAGT | AAGGTTAAGGCCGTTTTCCCT | Start: 63 |
| Tm | 57.975 | 59.854 | End: 199 |
| Size | 23 | 21 | 137 |
| Primer 4 details | |||
| Sequence | TCCCGTAGAAAGAAAGTACAATTGC | AGGTTAAGGCCGTTTTCCCT | Start: 69 |
| Tm | 59.59 | 59.224 | End: 198 |
| Size | 25 | 20 | 130 |
| Primer 5 details | |||
| Sequence | CCCGTAGAAAGAAAGTACAATTGCT | AGGTTAAGGCCGTTTTCCCT | Start: 70 |
| Tm | 59.588 | 59.224 | End: 198 |
| Size | 25 | 20 | 129 |
