ssr_primer
Primer Details for TRINITY_DN72268_c2_g1_i5 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGGACACTAGGGGTAGGT | CCCATCCCTTCCCCTTTGAC | Start: 163 |
| Tm | 60.034 | 60.033 | End: 426 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
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| Sequence | AACGTCGGACACTAGGGGTA | CCCATCCCTTCCCCTTTGAC | Start: 160 |
| Tm | 59.962 | 60.033 | End: 426 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
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| Sequence | TAACGTCGGACACTAGGGGT | CCCATCCCTTCCCCTTTGAC | Start: 159 |
| Tm | 59.962 | 60.033 | End: 426 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
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| Sequence | CTAGGGGTAGGTCTGCGACT | CCCATCCCTTCCCCTTTGAC | Start: 171 |
| Tm | 60.107 | 60.033 | End: 426 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
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| Sequence | GTCGGACACTAGGGGTAGGT | CGGTTTCCTCCCATCCCTTC | Start: 163 |
| Tm | 60.034 | 60.107 | End: 435 |
| Size | 20 | 20 | 273 |
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