ssr_primer
Primer Details for TRINITY_DN72231_c5_g5_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCGCCATTTCTTGTCGAC | TTTGCACAACTTTGGCAGCG | Start: 134 |
| Tm | 59.483 | 60.805 | End: 394 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | AAGCGCCATTTCTTGTCGAC | TTGCACAACTTTGGCAGCG | Start: 134 |
| Tm | 59.483 | 60.227 | End: 393 |
| Size | 20 | 19 | 260 |
| Primer 3 details |
|---|
| Sequence | AAGCGCCATTTCTTGTCGAC | GCACAACTTTGGCAGCGTT | Start: 134 |
| Tm | 59.483 | 60.227 | End: 391 |
| Size | 20 | 19 | 258 |
| Primer 4 details |
|---|
| Sequence | AAAGCGCCATTTCTTGTCGAC | TTTGCACAACTTTGGCAGCG | Start: 133 |
| Tm | 60.069 | 60.805 | End: 394 |
| Size | 21 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | CGCCATTTCTTGTCGACCAT | TTTGCACAACTTTGGCAGCG | Start: 137 |
| Tm | 58.913 | 60.805 | End: 394 |
| Size | 20 | 20 | 258 |
(A)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCGCCATTTCTTGTCGAC | TTTGCACAACTTTGGCAGCG | Start: 134 |
| Tm | 59.483 | 60.805 | End: 394 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | AAGCGCCATTTCTTGTCGAC | TTGCACAACTTTGGCAGCG | Start: 134 |
| Tm | 59.483 | 60.227 | End: 393 |
| Size | 20 | 19 | 260 |
| Primer 3 details |
|---|
| Sequence | AAGCGCCATTTCTTGTCGAC | GCACAACTTTGGCAGCGTT | Start: 134 |
| Tm | 59.483 | 60.227 | End: 391 |
| Size | 20 | 19 | 258 |
| Primer 4 details |
|---|
| Sequence | AAAGCGCCATTTCTTGTCGAC | TTTGCACAACTTTGGCAGCG | Start: 133 |
| Tm | 60.069 | 60.805 | End: 394 |
| Size | 21 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | CGCCATTTCTTGTCGACCAT | TTTGCACAACTTTGGCAGCG | Start: 137 |
| Tm | 58.913 | 60.805 | End: 394 |
| Size | 20 | 20 | 258 |
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