ssr_primer
Primer Details for TRINITY_DN72160_c1_g8_i3 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAGCGCAAGAACGATAA | GAAATGGAAGGGAGCACGGA | Start: 525 |
| Tm | 59.752 | 60.035 | End: 804 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | ACGCCTGTTCCCATCAATGA | GAAATGGAAGGGAGCACGGA | Start: 604 |
| Tm | 59.672 | 60.035 | End: 804 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
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| Sequence | CCCAGCGCAAGAACGATAAC | GAAATGGAAGGGAGCACGGA | Start: 526 |
| Tm | 59.626 | 60.035 | End: 804 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
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| Sequence | TTTGAGTGCAGTTCCAACGC | GAAATGGAAGGGAGCACGGA | Start: 588 |
| Tm | 59.62 | 60.035 | End: 804 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
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| Sequence | TCCCAGCGCAAGAACGATAA | TGGAAGGGAGCACGGAAAAA | Start: 525 |
| Tm | 59.752 | 59.816 | End: 800 |
| Size | 20 | 20 | 276 |
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