ssr_primer
Primer Details for TRINITY_DN72134_c2_g1_i1 |
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| (T)10acagaataaatttatctg(TCTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGTTGGCTTTTGCTTTG | GCCCTGGGTGGAATCCTAAC | Start: 410 |
| Tm | 59.82 | 60.107 | End: 589 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
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| Sequence | CAACTCCACGCACAAGTTGG | AGGCGTTGTTCATTCGGGTA | Start: 73 |
| Tm | 59.971 | 59.678 | End: 325 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
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| Sequence | TGGGGTTGGCTTTTGCTTTG | CATGCTGCCCTTGAAAGCTC | Start: 410 |
| Tm | 59.82 | 59.827 | End: 633 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
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| Sequence | TCACAACTCCACGCACAAGT | AGGCGTTGTTCATTCGGGTA | Start: 70 |
| Tm | 60.108 | 59.678 | End: 325 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
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| Sequence | TGGGGTTGGCTTTTGCTTTG | ATGCTGCCCTTGAAAGCTCA | Start: 410 |
| Tm | 59.82 | 60.251 | End: 632 |
| Size | 20 | 20 | 223 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGGTTGGCTTTTGCTTTG | GCCCTGGGTGGAATCCTAAC | Start: 410 |
| Tm | 59.82 | 60.107 | End: 589 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | CAACTCCACGCACAAGTTGG | AGGCGTTGTTCATTCGGGTA | Start: 73 |
| Tm | 59.971 | 59.678 | End: 325 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TGGGGTTGGCTTTTGCTTTG | CATGCTGCCCTTGAAAGCTC | Start: 410 |
| Tm | 59.82 | 59.827 | End: 633 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | TCACAACTCCACGCACAAGT | AGGCGTTGTTCATTCGGGTA | Start: 70 |
| Tm | 60.108 | 59.678 | End: 325 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | TGGGGTTGGCTTTTGCTTTG | ATGCTGCCCTTGAAAGCTCA | Start: 410 |
| Tm | 59.82 | 60.251 | End: 632 |
| Size | 20 | 20 | 223 |
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