ssr_primer
Primer Details for TRINITY_DN72120_c5_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGGGGCTTTCAAGGTAAC | CTCGTAAGCTTGGCACCTCA | Start: 422 |
| Tm | 59.963 | 60.037 | End: 657 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
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| Sequence | TTTTCGGGGGCTTTCAAGGT | CTCGTAAGCTTGGCACCTCA | Start: 419 |
| Tm | 60.106 | 60.037 | End: 657 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
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| Sequence | GGCTTTCAAGGTAACGTGGC | CTCGTAAGCTTGGCACCTCA | Start: 427 |
| Tm | 59.76 | 60.037 | End: 657 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
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| Sequence | TGAGGTGCCAAGCTTACGAG | GGTTGAACGACGAGGGATCA | Start: 638 |
| Tm | 60.037 | 59.754 | End: 871 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | TGAGGTGCCAAGCTTACGAG | GACCTTGGGTTGAACGACGA | Start: 638 |
| Tm | 60.037 | 60.25 | End: 878 |
| Size | 20 | 20 | 241 |
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