ssr_primer
Primer Details for TRINITY_DN72066_c5_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGATCTGCGGGCTGATTGA | ACACTCGTGATACTTCGGCC | Start: 56 |
| Tm | 59.894 | 59.827 | End: 159 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | ACGAAAGCCCCATGTCCAAA | ACACTCGTGATACTTCGGCC | Start: 32 |
| Tm | 60.179 | 59.827 | End: 159 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | TCTGCGGGCTGATTGATGAA | ACACTCGTGATACTTCGGCC | Start: 60 |
| Tm | 59.747 | 59.827 | End: 159 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACGAAAGCCCCATGTCCAAA | GGGAGGTGACGAATGACGAT | Start: 32 |
| Tm | 60.179 | 59.54 | End: 139 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
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| Sequence | ATCTGCGGGCTGATTGATGA | ACACTCGTGATACTTCGGCC | Start: 59 |
| Tm | 59.529 | 59.827 | End: 159 |
| Size | 20 | 20 | 101 |
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