ssr_primer
Primer Details for TRINITY_DN72041_c5_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACAGTGGATTGGCGCTTGT | GCTCCATTACGGCTTCCACT | Start: 487 |
| Tm | 59.964 | 60.108 | End: 648 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | CCCTCCCGGGCTACAAAATT | Start: 960 |
| Tm | 59.889 | 60.034 | End: 1084 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | CCCTCCCGGGCTACAAAATT | Start: 881 |
| Tm | 59.889 | 60.034 | End: 1084 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TGGATTGGCGCTTGTTACCT | GCTCCATTACGGCTTCCACT | Start: 492 |
| Tm | 59.962 | 60.108 | End: 648 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | ACATGCGGTCCATTTTTGCC | Start: 960 |
| Tm | 59.889 | 60.037 | End: 1103 |
| Size | 20 | 20 | 144 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACAGTGGATTGGCGCTTGT | GCTCCATTACGGCTTCCACT | Start: 487 |
| Tm | 59.964 | 60.108 | End: 648 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | CCCTCCCGGGCTACAAAATT | Start: 960 |
| Tm | 59.889 | 60.034 | End: 1084 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | CCCTCCCGGGCTACAAAATT | Start: 881 |
| Tm | 59.889 | 60.034 | End: 1084 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TGGATTGGCGCTTGTTACCT | GCTCCATTACGGCTTCCACT | Start: 492 |
| Tm | 59.962 | 60.108 | End: 648 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | TGCCCTTTCCCACGATTTGA | ACATGCGGTCCATTTTTGCC | Start: 960 |
| Tm | 59.889 | 60.037 | End: 1103 |
| Size | 20 | 20 | 144 |
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