ssr_primer
Primer Details for TRINITY_DN72031_c3_g2_i6 |
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| (A)39: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTGTGTGTGTGTTCCTGC | GGACCTGTGGAAAACCGGAT | Start: 260 |
| Tm | 59.899 | 59.962 | End: 528 |
| Size | 20 | 20 | 269 |
| Primer 2 details |
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| Sequence | ATCCGGTTTTCCACAGGTCC | AGGTGGTGAGGTTTGCTGAG | Start: 509 |
| Tm | 59.962 | 59.891 | End: 662 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | TGTGTTCCTGCGTGTGAAGA | GGACCTGTGGAAAACCGGAT | Start: 269 |
| Tm | 59.823 | 59.962 | End: 528 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | ATCCGGTTTTCCACAGGTCC | GGTGAGGTTTGCTGAGGGTT | Start: 509 |
| Tm | 59.962 | 60.179 | End: 658 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
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| Sequence | ATCCGGTTTTCCACAGGTCC | CAGGTGGTGAGGTTTGCTGA | Start: 509 |
| Tm | 59.962 | 60.179 | End: 663 |
| Size | 20 | 20 | 155 |
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