ssr_primer
Primer Details for TRINITY_DN72025_c2_g1_i7 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCCTGGCTTTGTAGCTG | GAGGACAAAAAGCTGCGCAA | Start: 630 |
| Tm | 60.036 | 59.969 | End: 772 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | CCTGGCTTTGTAGCTGGTCA | GAGGACAAAAAGCTGCGCAA | Start: 634 |
| Tm | 59.963 | 59.969 | End: 772 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | CTCGAACTTCTGGATCCCCG | CTTGTCCTGACTGGCTGGAG | Start: 1211 |
| Tm | 59.897 | 60.037 | End: 1425 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
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| Sequence | AGAGCCTGGCTTTGTAGCTG | TGCTGCCCCTCAACAAAAGA | Start: 630 |
| Tm | 60.036 | 60.106 | End: 830 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
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| Sequence | GTCCAAACAGGGGTGGTGAA | CTTGTCCTGACTGGCTGGAG | Start: 1167 |
| Tm | 60.106 | 60.037 | End: 1425 |
| Size | 20 | 20 | 259 |
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