ssr_primer
Primer Details for TRINITY_DN72003_c4_g4_i2 |
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| (T)39: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGGAAATAGGCACAAGCT | CAAGGGGCCTGCACAATTTT | Start: 334 |
| Tm | 59.365 | 59.602 | End: 506 |
| Size | 21 | 20 | 173 |
| Primer 2 details |
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| Sequence | GCGATCCGGAAAAAGACAAGT | CAAGGGGCCTGCACAATTTT | Start: 269 |
| Tm | 59.195 | 59.602 | End: 506 |
| Size | 21 | 20 | 238 |
| Primer 3 details |
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| Sequence | AGCAATGGAGGAAATAGGCACA | CAAGGGGCCTGCACAATTTT | Start: 329 |
| Tm | 60.025 | 59.602 | End: 506 |
| Size | 22 | 20 | 178 |
| Primer 4 details |
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| Sequence | TCCACTGATCCCAAATGAAGCA | CAAGGGGCCTGCACAATTTT | Start: 407 |
| Tm | 59.959 | 59.602 | End: 506 |
| Size | 22 | 20 | 100 |
| Primer 5 details |
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| Sequence | AGCGATCCGGAAAAAGACAAG | CAAGGGGCCTGCACAATTTT | Start: 268 |
| Tm | 58.923 | 59.602 | End: 506 |
| Size | 21 | 20 | 239 |
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