ssr_primer
Primer Details for TRINITY_DN71912_c1_g1_i1 |
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| (T)12aaggttccgccgcccacggaaacttccccccttcccctccccgcccctttcccgtccttgccccttccc(T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGCCCAAATTTTCGGGGG | TTTTTGCCCAAAAGTGCCCC | Start: 215 |
| Tm | 60.036 | 60.107 | End: 376 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | GTTCACAACCGTCGCCAAAA | TTTTTGCCCAAAAGTGCCCC | Start: 177 |
| Tm | 59.901 | 60.107 | End: 376 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
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| Sequence | GTCCTTGCCCCTTCCCTTTT | TTTTGGCGACGGTTGTGAAC | Start: 79 |
| Tm | 60.179 | 59.901 | End: 196 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | ACCGTCGCCAAAATTCAGGA | TTTTTGCCCAAAAGTGCCCC | Start: 184 |
| Tm | 60.251 | 60.107 | End: 376 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
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| Sequence | CTTGCCCAAATTTTCGGGGG | GCTGCAGCCTCCCAAAATTT | Start: 215 |
| Tm | 60.036 | 59.677 | End: 348 |
| Size | 20 | 20 | 134 |
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