ssr_primer
Primer Details for TRINITY_DN71903_c4_g4_i2 |
|---|
| (AATA)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGACCCAGAGCATAGCTG | CACCACACTCGAGATGCGTA | Start: 1140 |
| Tm | 59.968 | 59.83 | End: 1388 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | CTCGACCCAGAGCATAGCTG | ACCACACTCGAGATGCGTAC | Start: 1140 |
| Tm | 59.968 | 59.829 | End: 1387 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | GCAAATCTTGGCTACCAGCG | Start: 578 |
| Tm | 59.827 | 59.901 | End: 788 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | CGCAAATCTTGGCTACCAGC | Start: 578 |
| Tm | 59.827 | 59.901 | End: 789 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | TCTCGACCCAGAGCATAGCT | CACCACACTCGAGATGCGTA | Start: 1139 |
| Tm | 60.106 | 59.83 | End: 1388 |
| Size | 20 | 20 | 250 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGACCCAGAGCATAGCTG | CACCACACTCGAGATGCGTA | Start: 1140 |
| Tm | 59.968 | 59.83 | End: 1388 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | CTCGACCCAGAGCATAGCTG | ACCACACTCGAGATGCGTAC | Start: 1140 |
| Tm | 59.968 | 59.829 | End: 1387 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | GCAAATCTTGGCTACCAGCG | Start: 578 |
| Tm | 59.827 | 59.901 | End: 788 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | CGCAAATCTTGGCTACCAGC | Start: 578 |
| Tm | 59.827 | 59.901 | End: 789 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | TCTCGACCCAGAGCATAGCT | CACCACACTCGAGATGCGTA | Start: 1139 |
| Tm | 60.106 | 59.83 | End: 1388 |
| Size | 20 | 20 | 250 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGACCCAGAGCATAGCTG | CACCACACTCGAGATGCGTA | Start: 1140 |
| Tm | 59.968 | 59.83 | End: 1388 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | CTCGACCCAGAGCATAGCTG | ACCACACTCGAGATGCGTAC | Start: 1140 |
| Tm | 59.968 | 59.829 | End: 1387 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | GCAAATCTTGGCTACCAGCG | Start: 578 |
| Tm | 59.827 | 59.901 | End: 788 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | CGCAAATCTTGGCTACCAGC | Start: 578 |
| Tm | 59.827 | 59.901 | End: 789 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | TCTCGACCCAGAGCATAGCT | CACCACACTCGAGATGCGTA | Start: 1139 |
| Tm | 60.106 | 59.83 | End: 1388 |
| Size | 20 | 20 | 250 |
(A)10taaatttacaggtaaataaat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGACCCAGAGCATAGCTG | CACCACACTCGAGATGCGTA | Start: 1140 |
| Tm | 59.968 | 59.83 | End: 1388 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | CTCGACCCAGAGCATAGCTG | ACCACACTCGAGATGCGTAC | Start: 1140 |
| Tm | 59.968 | 59.829 | End: 1387 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | GCAAATCTTGGCTACCAGCG | Start: 578 |
| Tm | 59.827 | 59.901 | End: 788 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | TTGGCTCATGAGTCGTACGG | CGCAAATCTTGGCTACCAGC | Start: 578 |
| Tm | 59.827 | 59.901 | End: 789 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | TCTCGACCCAGAGCATAGCT | CACCACACTCGAGATGCGTA | Start: 1139 |
| Tm | 60.106 | 59.83 | End: 1388 |
| Size | 20 | 20 | 250 |
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