ssr_primer
Primer Details for TRINITY_DN71865_c1_g1_i5 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGATGCTACAGAGTGAT | GATCGTTCCGGGGTATGTCC | Start: 78 |
| Tm | 59.969 | 59.967 | End: 335 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | ATCAGGCGTGATTCGAGCAT | TGGTGCGGATGATGATCTCG | Start: 96 |
| Tm | 59.894 | 59.967 | End: 362 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | ATCAGGCGTGATTCGAGCAT | GATCGTTCCGGGGTATGTCC | Start: 96 |
| Tm | 59.894 | 59.967 | End: 335 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | CGGCGATGCTACAGAGTGAT | CCTCCATCATGGTGACGCTT | Start: 78 |
| Tm | 59.969 | 60.108 | End: 314 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | TCAGGCGTGATTCGAGCATT | TGGTGCGGATGATGATCTCG | Start: 97 |
| Tm | 60.108 | 59.967 | End: 362 |
| Size | 20 | 20 | 266 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGATGCTACAGAGTGAT | GATCGTTCCGGGGTATGTCC | Start: 78 |
| Tm | 59.969 | 59.967 | End: 335 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | ATCAGGCGTGATTCGAGCAT | TGGTGCGGATGATGATCTCG | Start: 96 |
| Tm | 59.894 | 59.967 | End: 362 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | ATCAGGCGTGATTCGAGCAT | GATCGTTCCGGGGTATGTCC | Start: 96 |
| Tm | 59.894 | 59.967 | End: 335 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | CGGCGATGCTACAGAGTGAT | CCTCCATCATGGTGACGCTT | Start: 78 |
| Tm | 59.969 | 60.108 | End: 314 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | TCAGGCGTGATTCGAGCATT | TGGTGCGGATGATGATCTCG | Start: 97 |
| Tm | 60.108 | 59.967 | End: 362 |
| Size | 20 | 20 | 266 |
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