ssr_primer
Primer Details for TRINITY_DN71853_c4_g1_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGGGGTGCAGAAACTTC | GGAACCCAACTCTCGAGAGC | Start: 191 |
| Tm | 59.964 | 60.109 | End: 306 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TACGGGGGTGCAGAAACTTC | CCTAACTGTCAAGCGCGTTG | Start: 191 |
| Tm | 59.964 | 59.836 | End: 366 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | TACGTAGGGAAGGGAGGAGC | GGAACCCAACTCTCGAGAGC | Start: 149 |
| Tm | 60.106 | 60.109 | End: 306 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | CGTACGTAGGGAAGGGAGGA | GGAACCCAACTCTCGAGAGC | Start: 147 |
| Tm | 60.107 | 60.109 | End: 306 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | GTGTAACGGGTGCGTACGTA | GGAACCCAACTCTCGAGAGC | Start: 135 |
| Tm | 60.11 | 60.109 | End: 306 |
| Size | 20 | 20 | 172 |
(C)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGGGGGTGCAGAAACTTC | GGAACCCAACTCTCGAGAGC | Start: 191 |
| Tm | 59.964 | 60.109 | End: 306 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TACGGGGGTGCAGAAACTTC | CCTAACTGTCAAGCGCGTTG | Start: 191 |
| Tm | 59.964 | 59.836 | End: 366 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | TACGTAGGGAAGGGAGGAGC | GGAACCCAACTCTCGAGAGC | Start: 149 |
| Tm | 60.106 | 60.109 | End: 306 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | CGTACGTAGGGAAGGGAGGA | GGAACCCAACTCTCGAGAGC | Start: 147 |
| Tm | 60.107 | 60.109 | End: 306 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | GTGTAACGGGTGCGTACGTA | GGAACCCAACTCTCGAGAGC | Start: 135 |
| Tm | 60.11 | 60.109 | End: 306 |
| Size | 20 | 20 | 172 |
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