ssr_primer
Primer Details for TRINITY_DN71792_c6_g2_i1 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACGCCGCCAAAATCTTTC | ACAGAAGTGTGGTAAGGCGG | Start: 280 |
| Tm | 60.456 | 59.965 | End: 483 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | GACGCCGCCAAAATCTTTCT | ACAGAAGTGTGGTAAGGCGG | Start: 281 |
| Tm | 59.478 | 59.965 | End: 483 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
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| Sequence | AAATGCCTAGGGTGGATGCC | GAAAGATTTTGGCGGCGTCC | Start: 140 |
| Tm | 60.106 | 60.456 | End: 299 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | AAATGCCTAGGGTGGATGCC | AGAAAGATTTTGGCGGCGTC | Start: 140 |
| Tm | 60.106 | 59.478 | End: 300 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | GGGAGAAATGCCTAGGGTGG | GAAAGATTTTGGCGGCGTCC | Start: 135 |
| Tm | 59.816 | 60.456 | End: 299 |
| Size | 20 | 20 | 165 |
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