ssr_primer
Primer Details for TRINITY_DN71770_c2_g2_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGAGCTGCCGTACACAAA | CAGAGGTGTGGCATGGTAGG | Start: 116 |
| Tm | 59.894 | 60.108 | End: 279 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
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| Sequence | ACAGAGCTGCCGTACACAAA | CCAGAGGTGTGGCATGGTAG | Start: 116 |
| Tm | 59.894 | 60.108 | End: 280 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
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| Sequence | TGGAAAGGTCGCCATTTGGT | GTTTTCTCTTGGCTCCCCGA | Start: 637 |
| Tm | 60.179 | 59.964 | End: 820 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
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| Sequence | TGGAAAGGTCGCCATTTGGT | AGTTTTCTCTTGGCTCCCCG | Start: 637 |
| Tm | 60.179 | 59.963 | End: 821 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
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| Sequence | ACCCCTTCTCTCCTTCCCAA | GTTTTCTCTTGGCTCCCCGA | Start: 609 |
| Tm | 59.804 | 59.964 | End: 820 |
| Size | 20 | 20 | 212 |
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