ssr_primer
Primer Details for TRINITY_DN71642_c3_g1_i2 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCCCTCCTCCGTTTTT | AGCACCGTTTTCCGCAAAAA | Start: 58 |
| Tm | 59.888 | 59.826 | End: 241 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
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| Sequence | TAGTTCCCCCTCCTCCGTTT | AGCACCGTTTTCCGCAAAAA | Start: 56 |
| Tm | 59.882 | 59.826 | End: 241 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
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| Sequence | TTTTTGCGGAAAACGGTGCT | CACACAGCGCATTTAAGGGG | Start: 222 |
| Tm | 59.826 | 59.829 | End: 355 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
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| Sequence | CGAGGTTAATGTCGCGGTCA | GCGGGGGTCATAAGTTCACA | Start: 527 |
| Tm | 60.457 | 60.036 | End: 762 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | CGAGGTTAATGTCGCGGTCA | TGCGGGGGTCATAAGTTCAC | Start: 527 |
| Tm | 60.457 | 60.036 | End: 763 |
| Size | 20 | 20 | 237 |
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