ssr_primer
Primer Details for TRINITY_DN71622_c4_g1_i1 |
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| (T)10attttgaaaaaatagatcgtacgtatcgactcttttttgaagcagt(G)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGGATGCGCTGACAAGTCA | TACGACCAATTTCGGCGGAA | Start: 524 |
| Tm | 59.965 | 60.038 | End: 767 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
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| Sequence | AAGGATGCGCTGACAAGTCA | TTACGACCAATTTCGGCGGA | Start: 524 |
| Tm | 59.965 | 60.038 | End: 768 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
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| Sequence | TCCGCCGAAATTGGTCGTAA | TTCTGGTATTCAGTGCGCGT | Start: 749 |
| Tm | 60.038 | 60.038 | End: 885 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | TTCCGCCGAAATTGGTCGTA | TTCTGGTATTCAGTGCGCGT | Start: 748 |
| Tm | 60.038 | 60.038 | End: 885 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
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| Sequence | GGTGAACCGCAAGCTGATTG | TGACTTGTCAGCGCATCCTT | Start: 275 |
| Tm | 60.11 | 59.965 | End: 543 |
| Size | 20 | 20 | 269 |
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