ssr_primer
Primer Details for TRINITY_DN71601_c4_g1_i5 |
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| (A)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGTCGACAACGGACTTG | TATACGCGGCTGATCGGAAC | Start: 1529 |
| Tm | 59.968 | 60.04 | End: 1666 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | AATGGCTCCCAGAACGACTG | GTGGTGCCTGAAGATGTGGA | Start: 1057 |
| Tm | 60.036 | 59.963 | End: 1268 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
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| Sequence | CAATGGCTCCCAGAACGACT | GTGGTGCCTGAAGATGTGGA | Start: 1056 |
| Tm | 60.036 | 59.963 | End: 1268 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
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| Sequence | GTGATAGCGGGTGGTGTGAA | AGTCGTTCTGGGAGCCATTG | Start: 956 |
| Tm | 60.037 | 60.036 | End: 1075 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | GTGATAGCGGGTGGTGTGAA | CAGTCGTTCTGGGAGCCATT | Start: 956 |
| Tm | 60.037 | 60.036 | End: 1076 |
| Size | 20 | 20 | 121 |
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