ssr_primer
Primer Details for TRINITY_DN71599_c3_g6_i1 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGCCGCATAACGTTCTT | ACCGTGACACTCTTCGGAAC | Start: 422 |
| Tm | 60.037 | 59.968 | End: 592 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | AGAGTTCGGCAGGTAGTTGC | GCTTCACACGACGAGAGGAA | Start: 1776 |
| Tm | 60.037 | 60.039 | End: 1954 |
| Size | 20 | 20 | 179 |
| Primer 3 details |
|---|
| Sequence | AGAGTTCGGCAGGTAGTTGC | ACTGCTTCACACGACGAGAG | Start: 1776 |
| Tm | 60.037 | 60.04 | End: 1957 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
|---|
| Sequence | CTGTATCCCGTAGCGTCGTC | GAGTCCTCATATGCCCGTCG | Start: 1063 |
| Tm | 60.041 | 60.039 | End: 1274 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | CTGTATCCCGTAGCGTCGTC | CGAGTCCTCATATGCCCGTC | Start: 1063 |
| Tm | 60.041 | 60.039 | End: 1275 |
| Size | 20 | 20 | 213 |
(T)10a(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGCCGCATAACGTTCTT | ACCGTGACACTCTTCGGAAC | Start: 422 |
| Tm | 60.037 | 59.968 | End: 592 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | AGAGTTCGGCAGGTAGTTGC | GCTTCACACGACGAGAGGAA | Start: 1776 |
| Tm | 60.037 | 60.039 | End: 1954 |
| Size | 20 | 20 | 179 |
| Primer 3 details |
|---|
| Sequence | AGAGTTCGGCAGGTAGTTGC | ACTGCTTCACACGACGAGAG | Start: 1776 |
| Tm | 60.037 | 60.04 | End: 1957 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
|---|
| Sequence | CTGTATCCCGTAGCGTCGTC | GAGTCCTCATATGCCCGTCG | Start: 1063 |
| Tm | 60.041 | 60.039 | End: 1274 |
| Size | 20 | 20 | 212 |
| Primer 5 details |
|---|
| Sequence | CTGTATCCCGTAGCGTCGTC | CGAGTCCTCATATGCCCGTC | Start: 1063 |
| Tm | 60.041 | 60.039 | End: 1275 |
| Size | 20 | 20 | 213 |
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