ssr_primer
Primer Details for TRINITY_DN71592_c3_g1_i1 |
|---|
| (GA)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGCTTAAATCTGACGTG | CCTTTTGTACCTTTCGCGGC | Start: 281 |
| Tm | 59.975 | 60.11 | End: 390 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | CACGTCAGATTTAAGCGCCG | Start: 166 |
| Tm | 59.885 | 59.975 | End: 300 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | TCCAACGGCAACATGGATGA | Start: 166 |
| Tm | 59.885 | 59.963 | End: 324 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GCCCGCCGAAGTAATTTTGG | CACGTCAGATTTAAGCGCCG | Start: 151 |
| Tm | 60.179 | 59.975 | End: 300 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | CGATTACCAACCTCTCGCGA | Start: 166 |
| Tm | 59.885 | 59.9 | End: 349 |
| Size | 20 | 20 | 184 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGCTTAAATCTGACGTG | CCTTTTGTACCTTTCGCGGC | Start: 281 |
| Tm | 59.975 | 60.11 | End: 390 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | CACGTCAGATTTAAGCGCCG | Start: 166 |
| Tm | 59.885 | 59.975 | End: 300 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | TCCAACGGCAACATGGATGA | Start: 166 |
| Tm | 59.885 | 59.963 | End: 324 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GCCCGCCGAAGTAATTTTGG | CACGTCAGATTTAAGCGCCG | Start: 151 |
| Tm | 60.179 | 59.975 | End: 300 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TTTGGGGAGAGAGAGGACGT | CGATTACCAACCTCTCGCGA | Start: 166 |
| Tm | 59.885 | 59.9 | End: 349 |
| Size | 20 | 20 | 184 |
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