ssr_primer
Primer Details for TRINITY_DN71592_c2_g1_i1 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTATGTCCACGGGATTCGC | GTTGGTCGGCAAGTTGAACC | Start: 258 |
| Tm | 59.896 | 59.97 | End: 423 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
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| Sequence | AGGGCAACGACTAAAGGCTC | GCGAATCCCGTGGACATAGT | Start: 22 |
| Tm | 60.036 | 59.896 | End: 277 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | AGGGCAACGACTAAAGGCTC | CGCATTTGAGCCATCGGTTT | Start: 22 |
| Tm | 60.036 | 59.829 | End: 158 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | AAACCGATGGCTCAAATGCG | GCGAATCCCGTGGACATAGT | Start: 139 |
| Tm | 59.829 | 59.896 | End: 277 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | AGGGCAACGACTAAAGGCTC | CTGAAAATCAGTGCAGCGCA | Start: 22 |
| Tm | 60.036 | 59.761 | End: 174 |
| Size | 20 | 20 | 153 |
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