ssr_primer
Primer Details for TRINITY_DN71560_c1_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTGCGAACACCCTCTTTA | TGGGGGATAAATGTACCGTGC | Start: 39 |
| Tm | 60.669 | 60.134 | End: 267 |
| Size | 20 | 21 | 229 |
| Primer 2 details |
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| Sequence | CGCTGCGAACACCCTCTTTA | GGGGGATAAATGTACCGTGCT | Start: 39 |
| Tm | 60.669 | 59.858 | End: 266 |
| Size | 20 | 21 | 228 |
| Primer 3 details |
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| Sequence | CGCTGCGAACACCCTCTTTA | ACCGTGCTACAAAGTTTCGGA | Start: 39 |
| Tm | 60.669 | 60.203 | End: 253 |
| Size | 20 | 21 | 215 |
| Primer 4 details |
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| Sequence | CGCTGCGAACACCCTCTTTA | ACGGCTATCAATACCAGTGGC | Start: 39 |
| Tm | 60.669 | 60.203 | End: 138 |
| Size | 20 | 21 | 100 |
| Primer 5 details |
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| Sequence | GCCACGCCACTGGTATTGAT | TGGGGGATAAATGTACCGTGC | Start: 113 |
| Tm | 60.748 | 60.134 | End: 267 |
| Size | 20 | 21 | 155 |
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