ssr_primer
Primer Details for TRINITY_DN71500_c1_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCCTTCAATTCGGAGGTT | GTCGGTCAATGTTTGGGTGC | Start: 451 |
| Tm | 59.964 | 60.04 | End: 644 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
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| Sequence | AACGCCTTCAATTCGGAGGT | GTCGGTCAATGTTTGGGTGC | Start: 450 |
| Tm | 59.964 | 60.04 | End: 644 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
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| Sequence | ATCTGCTGTTTTGGACCGGT | GTCGGTCAATGTTTGGGTGC | Start: 526 |
| Tm | 59.89 | 60.04 | End: 644 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | GTAGCCTACGCAGGAGTGTG | GTCGGTCAATGTTTGGGTGC | Start: 478 |
| Tm | 60.179 | 60.04 | End: 644 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGCCTTCAATTCGGAGGTT | ATCACTTCACTGCACCGGTC | Start: 451 |
| Tm | 59.964 | 60.321 | End: 558 |
| Size | 20 | 20 | 108 |
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