ssr_primer
Primer Details for TRINITY_DN71473_c2_g2_i2 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCCGACTTCATGGGGCA | CGTCGATTTTGATCGCACCC | Start: 333 |
| Tm | 59.889 | 59.974 | End: 516 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | TTTTCCGACTTCATGGGGCA | GCACTTTGTGGCTGGATGTG | Start: 333 |
| Tm | 59.889 | 60.039 | End: 456 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | GGCACCATGTGGAAAATGCA | CGTCGATTTTGATCGCACCC | Start: 349 |
| Tm | 59.679 | 59.974 | End: 516 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | CACATCCAGCCACAAAGTGC | GCCGCCATCTTTGATACTGC | Start: 437 |
| Tm | 60.039 | 59.691 | End: 623 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | TTTTCCGACTTCATGGGGCA | CGATTTTGATCGCACCCACG | Start: 333 |
| Tm | 59.889 | 60.248 | End: 513 |
| Size | 20 | 20 | 181 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCCGACTTCATGGGGCA | CGTCGATTTTGATCGCACCC | Start: 333 |
| Tm | 59.889 | 59.974 | End: 516 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | TTTTCCGACTTCATGGGGCA | GCACTTTGTGGCTGGATGTG | Start: 333 |
| Tm | 59.889 | 60.039 | End: 456 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | GGCACCATGTGGAAAATGCA | CGTCGATTTTGATCGCACCC | Start: 349 |
| Tm | 59.679 | 59.974 | End: 516 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | CACATCCAGCCACAAAGTGC | GCCGCCATCTTTGATACTGC | Start: 437 |
| Tm | 60.039 | 59.691 | End: 623 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | TTTTCCGACTTCATGGGGCA | CGATTTTGATCGCACCCACG | Start: 333 |
| Tm | 59.889 | 60.248 | End: 513 |
| Size | 20 | 20 | 181 |
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