ssr_primer
Primer Details for TRINITY_DN71453_c2_g1_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTAATTGCATGCGGGCTC | TGCTCCCGGAAATGAAGGTC | Start: 249 |
| Tm | 59.97 | 60.035 | End: 391 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CAGTAATTGCATGCGGGCTC | GTCCGTGCTGCATTGGAATC | Start: 249 |
| Tm | 59.97 | 59.901 | End: 522 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GACCTTCATTTCCGGGAGCA | GTCCGTGCTGCATTGGAATC | Start: 372 |
| Tm | 60.035 | 59.901 | End: 522 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | CTCTTAACATCCCGTCGCGA | TGCTCCCGGAAATGAAGGTC | Start: 266 |
| Tm | 59.9 | 60.035 | End: 391 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CAGTAATTGCATGCGGGCTC | GGTCCCTTCTCCGAGATCCT | Start: 249 |
| Tm | 59.97 | 60.106 | End: 375 |
| Size | 20 | 20 | 127 |
(T)10attggaga(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTAATTGCATGCGGGCTC | TGCTCCCGGAAATGAAGGTC | Start: 249 |
| Tm | 59.97 | 60.035 | End: 391 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CAGTAATTGCATGCGGGCTC | GTCCGTGCTGCATTGGAATC | Start: 249 |
| Tm | 59.97 | 59.901 | End: 522 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GACCTTCATTTCCGGGAGCA | GTCCGTGCTGCATTGGAATC | Start: 372 |
| Tm | 60.035 | 59.901 | End: 522 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | CTCTTAACATCCCGTCGCGA | TGCTCCCGGAAATGAAGGTC | Start: 266 |
| Tm | 59.9 | 60.035 | End: 391 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CAGTAATTGCATGCGGGCTC | GGTCCCTTCTCCGAGATCCT | Start: 249 |
| Tm | 59.97 | 60.106 | End: 375 |
| Size | 20 | 20 | 127 |
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