ssr_primer
Primer Details for TRINITY_DN71435_c6_g1_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGATTGCCCGTTCGACAA | TCGGAACTTACCCGTTCTGC | Start: 389 |
| Tm | 60.038 | 60.038 | End: 538 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | TACGATTGCCCGTTCGACAA | TCGATTCGTCGCGATGTCTT | Start: 389 |
| Tm | 60.038 | 59.902 | End: 561 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | AAAGCGCGCTCGAGAAAATC | TCGATTCGTCGCGATGTCTT | Start: 462 |
| Tm | 59.905 | 59.902 | End: 561 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | GAAAGCGCGCTCGAGAAAAT | TCGATTCGTCGCGATGTCTT | Start: 461 |
| Tm | 59.905 | 59.902 | End: 561 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | TACGATTGCCCGTTCGACAA | AAAGGTCGATTCGTCGCGAT | Start: 389 |
| Tm | 60.038 | 60.179 | End: 566 |
| Size | 20 | 20 | 178 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACGATTGCCCGTTCGACAA | TCGGAACTTACCCGTTCTGC | Start: 389 |
| Tm | 60.038 | 60.038 | End: 538 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | TACGATTGCCCGTTCGACAA | TCGATTCGTCGCGATGTCTT | Start: 389 |
| Tm | 60.038 | 59.902 | End: 561 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | AAAGCGCGCTCGAGAAAATC | TCGATTCGTCGCGATGTCTT | Start: 462 |
| Tm | 59.905 | 59.902 | End: 561 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | GAAAGCGCGCTCGAGAAAAT | TCGATTCGTCGCGATGTCTT | Start: 461 |
| Tm | 59.905 | 59.902 | End: 561 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | TACGATTGCCCGTTCGACAA | AAAGGTCGATTCGTCGCGAT | Start: 389 |
| Tm | 60.038 | 60.179 | End: 566 |
| Size | 20 | 20 | 178 |
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