ssr_primer
Primer Details for TRINITY_DN71435_c5_g1_i2 |
|---|
| (A)29: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTAATGCCGTGCCCAAAAA | ATCGAGCAGCGCTATTTCGA | Start: 2 |
| Tm | 60.038 | 59.969 | End: 281 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TGTACTCTTGGCTTCAGCGG | GAGGCACAAGAGCACTGAGT | Start: 1436 |
| Tm | 60.037 | 59.965 | End: 1612 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | ACTCAGTGCTCTTGTGCCTC | AATAAGCCACACGAGCACGA | Start: 1593 |
| Tm | 59.965 | 60.038 | End: 1773 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | TCGTGCTCGTGTGGCTTATT | GGATTGCCCAAGAGAGCACT | Start: 1754 |
| Tm | 60.038 | 60.035 | End: 1985 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | TCGTGCTCGTGTGGCTTATT | ACTGGATTGCCCAAGAGAGC | Start: 1754 |
| Tm | 60.038 | 60.035 | End: 1988 |
| Size | 20 | 20 | 235 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTAATGCCGTGCCCAAAAA | ATCGAGCAGCGCTATTTCGA | Start: 2 |
| Tm | 60.038 | 59.969 | End: 281 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
|---|
| Sequence | TGTACTCTTGGCTTCAGCGG | GAGGCACAAGAGCACTGAGT | Start: 1436 |
| Tm | 60.037 | 59.965 | End: 1612 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | ACTCAGTGCTCTTGTGCCTC | AATAAGCCACACGAGCACGA | Start: 1593 |
| Tm | 59.965 | 60.038 | End: 1773 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | TCGTGCTCGTGTGGCTTATT | GGATTGCCCAAGAGAGCACT | Start: 1754 |
| Tm | 60.038 | 60.035 | End: 1985 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | TCGTGCTCGTGTGGCTTATT | ACTGGATTGCCCAAGAGAGC | Start: 1754 |
| Tm | 60.038 | 60.035 | End: 1988 |
| Size | 20 | 20 | 235 |
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