ssr_primer
Primer Details for TRINITY_DN71424_c1_g1_i4 |
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| (T)12ctatcaatgaatgaatttagaatttcatcatgagaaagtgctgagaaa(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGTGTCCTTCGACTTGC | TGGCTTTTGACTGGGCGTAT | Start: 1571 |
| Tm | 59.966 | 59.962 | End: 1671 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | TAACAGTAATGCGCACGGGT | AAACCTGCGTTTCCGAGCTA | Start: 1121 |
| Tm | 60.037 | 59.966 | End: 1296 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | GTTACACAGGTCGACGCTGA | AGTTCTTGAAGGAGGCGAGC | Start: 128 |
| Tm | 60.04 | 60.037 | End: 381 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
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| Sequence | TGAGGTGTCCTTCGACTTGC | CAGTTCGGAGCTGAGGTGAG | Start: 1571 |
| Tm | 59.966 | 60.109 | End: 1832 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | GCTCGCCTCCTTCAAGAACT | CATCTTCTGGAGCCTGGTGG | Start: 362 |
| Tm | 60.037 | 60.107 | End: 497 |
| Size | 20 | 20 | 136 |
(A)17c(A)38: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGTGTCCTTCGACTTGC | TGGCTTTTGACTGGGCGTAT | Start: 1571 |
| Tm | 59.966 | 59.962 | End: 1671 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TAACAGTAATGCGCACGGGT | AAACCTGCGTTTCCGAGCTA | Start: 1121 |
| Tm | 60.037 | 59.966 | End: 1296 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | GTTACACAGGTCGACGCTGA | AGTTCTTGAAGGAGGCGAGC | Start: 128 |
| Tm | 60.04 | 60.037 | End: 381 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | TGAGGTGTCCTTCGACTTGC | CAGTTCGGAGCTGAGGTGAG | Start: 1571 |
| Tm | 59.966 | 60.109 | End: 1832 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | GCTCGCCTCCTTCAAGAACT | CATCTTCTGGAGCCTGGTGG | Start: 362 |
| Tm | 60.037 | 60.107 | End: 497 |
| Size | 20 | 20 | 136 |
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