ssr_primer
Primer Details for TRINITY_DN71344_c7_g1_i1 |
|---|
| (T)26: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTACAGTACACCAGCCGC | CACGCAACCACAGACTTTGG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2401 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | AACTACAGTACACCAGCCGC | CCACGCAACCACAGACTTTG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2402 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GCAAGTTGAGCCCAAAGTCG | GCACGCCTAGACGACTACTC | Start: 751 |
| Tm | 60.04 | 59.972 | End: 1015 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CACGCAACCACAGACTTTGG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2401 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CCACGCAACCACAGACTTTG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2402 |
| Size | 20 | 20 | 105 |
(TTTC)5(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTACAGTACACCAGCCGC | CACGCAACCACAGACTTTGG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2401 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | AACTACAGTACACCAGCCGC | CCACGCAACCACAGACTTTG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2402 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GCAAGTTGAGCCCAAAGTCG | GCACGCCTAGACGACTACTC | Start: 751 |
| Tm | 60.04 | 59.972 | End: 1015 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CACGCAACCACAGACTTTGG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2401 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CCACGCAACCACAGACTTTG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2402 |
| Size | 20 | 20 | 105 |
(T)11aatatttcgaaataacttattcaggcagtggattcagcctgtactggtcattctgatgaaatcgaacta(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTACAGTACACCAGCCGC | CACGCAACCACAGACTTTGG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2401 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | AACTACAGTACACCAGCCGC | CCACGCAACCACAGACTTTG | Start: 2268 |
| Tm | 60.038 | 59.971 | End: 2402 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | GCAAGTTGAGCCCAAAGTCG | GCACGCCTAGACGACTACTC | Start: 751 |
| Tm | 60.04 | 59.972 | End: 1015 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CACGCAACCACAGACTTTGG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2401 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | CACATGACGAAAAACGCGGT | CCACGCAACCACAGACTTTG | Start: 2298 |
| Tm | 60.042 | 59.971 | End: 2402 |
| Size | 20 | 20 | 105 |
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