ssr_primer
Primer Details for TRINITY_DN71317_c10_g1_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCATGTCACGACGGAGAT | GGTGGCACATGCATGTTCTC | Start: 433 |
| Tm | 59.899 | 59.828 | End: 622 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AGGCCTTTACCTCGTCATGC | GGTGGCACATGCATGTTCTC | Start: 391 |
| Tm | 60.108 | 59.828 | End: 622 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GCTCATGTCACGACGGAGAT | AGGTGGCACATGCATGTTCT | Start: 433 |
| Tm | 59.899 | 60.251 | End: 623 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | AGGCCTTTACCTCGTCATGC | AGGTGGCACATGCATGTTCT | Start: 391 |
| Tm | 60.108 | 60.251 | End: 623 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | CCTCGTCATGCGTGTATCGT | GGTGGCACATGCATGTTCTC | Start: 400 |
| Tm | 60.248 | 59.828 | End: 622 |
| Size | 20 | 20 | 223 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCATGTCACGACGGAGAT | GGTGGCACATGCATGTTCTC | Start: 433 |
| Tm | 59.899 | 59.828 | End: 622 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AGGCCTTTACCTCGTCATGC | GGTGGCACATGCATGTTCTC | Start: 391 |
| Tm | 60.108 | 59.828 | End: 622 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GCTCATGTCACGACGGAGAT | AGGTGGCACATGCATGTTCT | Start: 433 |
| Tm | 59.899 | 60.251 | End: 623 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | AGGCCTTTACCTCGTCATGC | AGGTGGCACATGCATGTTCT | Start: 391 |
| Tm | 60.108 | 60.251 | End: 623 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | CCTCGTCATGCGTGTATCGT | GGTGGCACATGCATGTTCTC | Start: 400 |
| Tm | 60.248 | 59.828 | End: 622 |
| Size | 20 | 20 | 223 |
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