ssr_primer
Primer Details for TRINITY_DN71262_c4_g4_i1 |
|---|
| (C)16actaaagtgggcaccttcaaaatttcaagttgaaatgaaagacgtggaca(C)11tcctctccaaattaatgttgaaagtgcgatctacttatatatcaagattaattttaggccgaaaaattggaggg(T)10ccgttagaatttgagttcaaaaaatcctttacttagtactacaaactcaaaacttttaaaaatta(T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGGTCGCTGCCACATG | GGACGGCCCCTAATGTCAAA | Start: 633 |
| Tm | 59.968 | 60.035 | End: 824 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
|---|
| Sequence | AAGTTCAGGAGGCTGCCTTC | CATGTGGCAGCGACCAAAAA | Start: 469 |
| Tm | 59.963 | 59.968 | End: 652 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | GAAAGTTCAGGAGGCTGCCT | CATGTGGCAGCGACCAAAAA | Start: 467 |
| Tm | 59.963 | 59.968 | End: 652 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | ATGAAAGACGTGGACACCCC | ACCCTCCAATTTTTCGGCCT | Start: 96 |
| Tm | 59.964 | 59.886 | End: 197 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | ATGAAAGACGTGGACACCCC | TACTGTTCCCTGTAGGCCCA | Start: 96 |
| Tm | 59.964 | 59.882 | End: 320 |
| Size | 20 | 20 | 225 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGGTCGCTGCCACATG | GGACGGCCCCTAATGTCAAA | Start: 633 |
| Tm | 59.968 | 60.035 | End: 824 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
|---|
| Sequence | AAGTTCAGGAGGCTGCCTTC | CATGTGGCAGCGACCAAAAA | Start: 469 |
| Tm | 59.963 | 59.968 | End: 652 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
|---|
| Sequence | GAAAGTTCAGGAGGCTGCCT | CATGTGGCAGCGACCAAAAA | Start: 467 |
| Tm | 59.963 | 59.968 | End: 652 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | ATGAAAGACGTGGACACCCC | ACCCTCCAATTTTTCGGCCT | Start: 96 |
| Tm | 59.964 | 59.886 | End: 197 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | ATGAAAGACGTGGACACCCC | TACTGTTCCCTGTAGGCCCA | Start: 96 |
| Tm | 59.964 | 59.882 | End: 320 |
| Size | 20 | 20 | 225 |
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