ssr_primer
Primer Details for TRINITY_DN71249_c3_g1_i3 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGGGTTTCCCTGGCACT | ACGTCAGCAGACTCTCTCCT | Start: 1419 |
| Tm | 60.032 | 59.962 | End: 1657 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
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| Sequence | CTCGCCACCAACCTTCATCT | ATGGACCCCAGCAGCTTTAC | Start: 1738 |
| Tm | 60.036 | 60.034 | End: 1876 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | CTCGCCACCAACCTTCATCT | CATGGACCCCAGCAGCTTTA | Start: 1738 |
| Tm | 60.036 | 60.034 | End: 1877 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
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| Sequence | CTCGCCACCAACCTTCATCT | CACACCAGGGTACATGGACC | Start: 1738 |
| Tm | 60.036 | 60.035 | End: 1889 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | AGGAGAGAGTCTGCTGACGT | ATGGACCCCAGCAGCTTTAC | Start: 1638 |
| Tm | 59.962 | 60.034 | End: 1876 |
| Size | 20 | 20 | 239 |
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