ssr_primer
Primer Details for TRINITY_DN71213_c5_g1_i2 |
|---|
| (G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGATGATTGGGACAGCC | TGCCTGGAACGTTTTGGAGT | Start: 48 |
| Tm | 60.108 | 60.107 | End: 183 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ACGATGATTGGGACAGCCTC | TGCCTGGAACGTTTTGGAGT | Start: 50 |
| Tm | 59.821 | 60.107 | End: 183 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | GACGATGATTGGGACAGCCT | TGCCTGGAACGTTTTGGAGT | Start: 49 |
| Tm | 59.821 | 60.107 | End: 183 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | TGACGATGATTGGGACAGCC | GCCTGGAACGTTTTGGAGTG | Start: 48 |
| Tm | 60.108 | 59.689 | End: 182 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TGACGATGATTGGGACAGCC | TGGTTTTAAGCCCCTCCTCG | Start: 48 |
| Tm | 60.108 | 59.673 | End: 164 |
| Size | 20 | 20 | 117 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGATGATTGGGACAGCC | TGCCTGGAACGTTTTGGAGT | Start: 48 |
| Tm | 60.108 | 60.107 | End: 183 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ACGATGATTGGGACAGCCTC | TGCCTGGAACGTTTTGGAGT | Start: 50 |
| Tm | 59.821 | 60.107 | End: 183 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | GACGATGATTGGGACAGCCT | TGCCTGGAACGTTTTGGAGT | Start: 49 |
| Tm | 59.821 | 60.107 | End: 183 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | TGACGATGATTGGGACAGCC | GCCTGGAACGTTTTGGAGTG | Start: 48 |
| Tm | 60.108 | 59.689 | End: 182 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TGACGATGATTGGGACAGCC | TGGTTTTAAGCCCCTCCTCG | Start: 48 |
| Tm | 60.108 | 59.673 | End: 164 |
| Size | 20 | 20 | 117 |
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