ssr_primer
Primer Details for TRINITY_DN71203_c2_g1_i10 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGGGCCCCTGATATGTT | CAAGCATTTTTGAGGGCCCC | Start: 56 |
| Tm | 60.029 | 60.036 | End: 282 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | AAGGAGGGCCCCTGATATGT | CAAGCATTTTTGAGGGCCCC | Start: 55 |
| Tm | 60.029 | 60.036 | End: 282 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
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| Sequence | GGGGCCCTCAAAAATGCTTG | ATCTCCACAGTTTCCTGCCG | Start: 263 |
| Tm | 60.036 | 60.036 | End: 410 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
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| Sequence | CGCTGCCATCTGCCATTTTT | ATCTCCACAGTTTCCTGCCG | Start: 218 |
| Tm | 60.109 | 60.036 | End: 410 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
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| Sequence | CCTAAGGAGGGCCCCTGATA | CAAGCATTTTTGAGGGCCCC | Start: 52 |
| Tm | 59.806 | 60.036 | End: 282 |
| Size | 20 | 20 | 231 |
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