ssr_primer
Primer Details for TRINITY_DN71174_c5_g2_i1 |
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| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGGGCCGGATTTACCTA | GCAGCGGCGGAAGTTAAAAT | Start: 712 |
| Tm | 60.031 | 59.83 | End: 839 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | CCAGGGCCGGATTTACCTAC | GCAGCGGCGGAAGTTAAAAT | Start: 713 |
| Tm | 59.892 | 59.83 | End: 839 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | TCCAGGGCCGGATTTACCTA | ATTGCGTCAAACACAGTGCG | Start: 712 |
| Tm | 60.031 | 60.317 | End: 870 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | TCGTGGGTTGGCTTATTCGT | TAGGTAAATCCGGCCCTGGA | Start: 532 |
| Tm | 59.678 | 60.031 | End: 731 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | CCAGGGCCGGATTTACCTAC | ATTGCGTCAAACACAGTGCG | Start: 713 |
| Tm | 59.892 | 60.317 | End: 870 |
| Size | 20 | 20 | 158 |
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