ssr_primer
Primer Details for TRINITY_DN71131_c1_g2_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGTGAAGGAGTGTGAAGA | AGCTCTTCTCTGTTTGCAGCT | Start: 0 |
| Tm | 59.966 | 59.928 | End: 162 |
| Size | 20 | 21 | 163 |
| Primer 2 details |
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| Sequence | AGGCGCAACTTCTCTGACTC | AGCTCTTCTCTGTTTGCAGCT | Start: 38 |
| Tm | 60.038 | 59.928 | End: 162 |
| Size | 20 | 21 | 125 |
| Primer 3 details |
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| Sequence | GAGGCGCAACTTCTCTGACT | AGCTCTTCTCTGTTTGCAGCT | Start: 37 |
| Tm | 60.038 | 59.928 | End: 162 |
| Size | 20 | 21 | 126 |
| Primer 4 details |
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| Sequence | ATTGACTGGAGGCGCAACTT | AGCTCTTCTCTGTTTGCAGCT | Start: 29 |
| Tm | 60.251 | 59.928 | End: 162 |
| Size | 20 | 21 | 134 |
| Primer 5 details |
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| Sequence | CCCATGGCATATTTGGGGTG | GCATACGGCTCTTTCATGGC | Start: 264 |
| Tm | 58.95 | 59.691 | End: 451 |
| Size | 20 | 20 | 188 |
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