ssr_primer
Primer Details for TRINITY_DN70991_c2_g3_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCTCCAGGTTCAGAGGC | CCGGTCATGGGAGCCATAAT | Start: 34 |
| Tm | 60.035 | 59.598 | End: 138 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | CGGTCTCTGAGTCTGGAACG | TGGGACTTTCATTGCGGGAT | Start: 84 |
| Tm | 59.831 | 59.669 | End: 358 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
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| Sequence | AGCTTCGATTGCTCCAGGTT | GCCATAATTACAGGCGCACC | Start: 27 |
| Tm | 59.674 | 59.689 | End: 126 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | GGTGCGCCTGTAATTATGGC | TGGGACTTTCATTGCGGGAT | Start: 107 |
| Tm | 59.689 | 59.669 | End: 358 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
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| Sequence | CGGTCTCTGAGTCTGGAACG | TGGGGTGGGACTTTCATTGC | Start: 84 |
| Tm | 59.831 | 60.542 | End: 363 |
| Size | 20 | 20 | 280 |
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