ssr_primer
Primer Details for TRINITY_DN70976_c3_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGACGGGAAGAGGGTTA | TGTTCAAGCTCTGACGCACA | Start: 1699 |
| Tm | 59.956 | 60.179 | End: 1932 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | AGGCACGCAAGATGAAAGGA | ACCCAACAGGAGGAGTACCA | Start: 4437 |
| Tm | 59.963 | 59.807 | End: 4666 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | TGGTACTCCTCCTGTTGGGT | AGGAACTCGTGCTGACATGG | Start: 4647 |
| Tm | 59.807 | 60.037 | End: 4873 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | ATGGGACGGGAAGAGGGTTA | TAAGGTGTGCAAGTCAGCCC | Start: 1699 |
| Tm | 59.956 | 60.251 | End: 1965 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | ATGCGGTTGATGACACCCAT | AGGCGACAGGTGATTTTCGA | Start: 2916 |
| Tm | 60.034 | 59.68 | End: 3126 |
| Size | 20 | 20 | 211 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGACGGGAAGAGGGTTA | TGTTCAAGCTCTGACGCACA | Start: 1699 |
| Tm | 59.956 | 60.179 | End: 1932 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | AGGCACGCAAGATGAAAGGA | ACCCAACAGGAGGAGTACCA | Start: 4437 |
| Tm | 59.963 | 59.807 | End: 4666 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | TGGTACTCCTCCTGTTGGGT | AGGAACTCGTGCTGACATGG | Start: 4647 |
| Tm | 59.807 | 60.037 | End: 4873 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | ATGGGACGGGAAGAGGGTTA | TAAGGTGTGCAAGTCAGCCC | Start: 1699 |
| Tm | 59.956 | 60.251 | End: 1965 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | ATGCGGTTGATGACACCCAT | AGGCGACAGGTGATTTTCGA | Start: 2916 |
| Tm | 60.034 | 59.68 | End: 3126 |
| Size | 20 | 20 | 211 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGACGGGAAGAGGGTTA | TGTTCAAGCTCTGACGCACA | Start: 1699 |
| Tm | 59.956 | 60.179 | End: 1932 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | AGGCACGCAAGATGAAAGGA | ACCCAACAGGAGGAGTACCA | Start: 4437 |
| Tm | 59.963 | 59.807 | End: 4666 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | TGGTACTCCTCCTGTTGGGT | AGGAACTCGTGCTGACATGG | Start: 4647 |
| Tm | 59.807 | 60.037 | End: 4873 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | ATGGGACGGGAAGAGGGTTA | TAAGGTGTGCAAGTCAGCCC | Start: 1699 |
| Tm | 59.956 | 60.251 | End: 1965 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | ATGCGGTTGATGACACCCAT | AGGCGACAGGTGATTTTCGA | Start: 2916 |
| Tm | 60.034 | 59.68 | End: 3126 |
| Size | 20 | 20 | 211 |
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