ssr_primer
Primer Details for TRINITY_DN70974_c5_g1_i5 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGAGGCGAGGGAAAATGA | GAATCAGGGCTCACGTGGAA | Start: 185 |
| Tm | 60.035 | 60.036 | End: 340 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
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| Sequence | AGATTCCTATCGGCTGTGCG | CTGTCCCGCAATACCCCTAC | Start: 248 |
| Tm | 59.967 | 59.894 | End: 486 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
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| Sequence | AGATTCCTATCGGCTGTGCG | CTCCCCTTGGATTCTGCCTG | Start: 248 |
| Tm | 59.967 | 60.107 | End: 503 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
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| Sequence | TTCCACGTGAGCCCTGATTC | CTGTCCCGCAATACCCCTAC | Start: 321 |
| Tm | 60.036 | 59.894 | End: 486 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
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| Sequence | GGTGAGGCGAGGGAAAATGA | ATCAGGGCTCACGTGGAATG | Start: 185 |
| Tm | 60.035 | 60.108 | End: 338 |
| Size | 20 | 20 | 154 |
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