ssr_primer
Primer Details for TRINITY_DN70864_c4_g5_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGGTTGAACATGGGTCCA | TGGTGAAGCGTTGCCAAATG | Start: 516 |
| Tm | 59.964 | 59.968 | End: 681 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CGAGGTTGAACATGGGTCCA | GTGAAGCGTTGCCAAATGGT | Start: 516 |
| Tm | 59.964 | 59.968 | End: 679 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | CTGCACTGAAAAACGGCTCC | TGGTGAAGCGTTGCCAAATG | Start: 547 |
| Tm | 60.04 | 59.968 | End: 681 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CTGCACTGAAAAACGGCTCC | GTGAAGCGTTGCCAAATGGT | Start: 547 |
| Tm | 60.04 | 59.968 | End: 679 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | CAGTGCTTTTTCGGTGGCTC | TGGACCCATGTTCAACCTCG | Start: 321 |
| Tm | 60.04 | 59.964 | End: 535 |
| Size | 20 | 20 | 215 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGGTTGAACATGGGTCCA | TGGTGAAGCGTTGCCAAATG | Start: 516 |
| Tm | 59.964 | 59.968 | End: 681 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CGAGGTTGAACATGGGTCCA | GTGAAGCGTTGCCAAATGGT | Start: 516 |
| Tm | 59.964 | 59.968 | End: 679 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | CTGCACTGAAAAACGGCTCC | TGGTGAAGCGTTGCCAAATG | Start: 547 |
| Tm | 60.04 | 59.968 | End: 681 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CTGCACTGAAAAACGGCTCC | GTGAAGCGTTGCCAAATGGT | Start: 547 |
| Tm | 60.04 | 59.968 | End: 679 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | CAGTGCTTTTTCGGTGGCTC | TGGACCCATGTTCAACCTCG | Start: 321 |
| Tm | 60.04 | 59.964 | End: 535 |
| Size | 20 | 20 | 215 |
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