ssr_primer
Primer Details for TRINITY_DN70859_c3_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATGGCATGACGCACACACT | GAAGTCTGCCCCAACTCCTC | Start: 1575 |
| Tm | 60.036 | 60.036 | End: 1768 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | GTATGGCATGACGCACACAC | GAAGTCTGCCCCAACTCCTC | Start: 1574 |
| Tm | 59.903 | 60.036 | End: 1768 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | GAGGAGTTGGGGCAGACTTC | TGGTGACAAAGAAGGTCGGG | Start: 1749 |
| Tm | 60.036 | 59.892 | End: 1967 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | ACTTCCGTCAAACCTGCCAT | TGGTGACAAAGAAGGTCGGG | Start: 1764 |
| Tm | 59.89 | 59.892 | End: 1967 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | TGTATGGCATGACGCACACA | GAAGTCTGCCCCAACTCCTC | Start: 1573 |
| Tm | 60.321 | 60.036 | End: 1768 |
| Size | 20 | 20 | 196 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATGGCATGACGCACACACT | GAAGTCTGCCCCAACTCCTC | Start: 1575 |
| Tm | 60.036 | 60.036 | End: 1768 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | GTATGGCATGACGCACACAC | GAAGTCTGCCCCAACTCCTC | Start: 1574 |
| Tm | 59.903 | 60.036 | End: 1768 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | GAGGAGTTGGGGCAGACTTC | TGGTGACAAAGAAGGTCGGG | Start: 1749 |
| Tm | 60.036 | 59.892 | End: 1967 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | ACTTCCGTCAAACCTGCCAT | TGGTGACAAAGAAGGTCGGG | Start: 1764 |
| Tm | 59.89 | 59.892 | End: 1967 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
|---|
| Sequence | TGTATGGCATGACGCACACA | GAAGTCTGCCCCAACTCCTC | Start: 1573 |
| Tm | 60.321 | 60.036 | End: 1768 |
| Size | 20 | 20 | 196 |
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