ssr_primer
Primer Details for TRINITY_DN70811_c1_g7_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTCATTCGATCGGGCAT | TTGTTACAGCGCACAAAGGC | Start: 410 |
| Tm | 59.964 | 59.97 | End: 645 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | TTCCGCAACTGCAGCTGATA | TTGTTACAGCGCACAAAGGC | Start: 491 |
| Tm | 60.036 | 59.97 | End: 645 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | TCCTCCATTGTTGGGTTCCG | TTGTTACAGCGCACAAAGGC | Start: 476 |
| Tm | 59.962 | 59.97 | End: 645 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TTGGGTGGAATCCGATTGCA | TTGTTACAGCGCACAAAGGC | Start: 385 |
| Tm | 59.961 | 59.97 | End: 645 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TGGAATCCGATTGCAGGCAT | TTGTTACAGCGCACAAAGGC | Start: 390 |
| Tm | 60.107 | 59.97 | End: 645 |
| Size | 20 | 20 | 256 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTCATTCGATCGGGCAT | TTGTTACAGCGCACAAAGGC | Start: 410 |
| Tm | 59.964 | 59.97 | End: 645 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | TTCCGCAACTGCAGCTGATA | TTGTTACAGCGCACAAAGGC | Start: 491 |
| Tm | 60.036 | 59.97 | End: 645 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | TCCTCCATTGTTGGGTTCCG | TTGTTACAGCGCACAAAGGC | Start: 476 |
| Tm | 59.962 | 59.97 | End: 645 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TTGGGTGGAATCCGATTGCA | TTGTTACAGCGCACAAAGGC | Start: 385 |
| Tm | 59.961 | 59.97 | End: 645 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TGGAATCCGATTGCAGGCAT | TTGTTACAGCGCACAAAGGC | Start: 390 |
| Tm | 60.107 | 59.97 | End: 645 |
| Size | 20 | 20 | 256 |
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